Day Three: Yet Again

Day three, SDS-PAGE (yet again). Checked on my 2nd electrophoretogram (from Day Two). It's better than the last one (from Day One), no bowling alleys but still not good. :(

Anyway, today's SDS-PAGEing went quite well, probably the result of our two days of practice. I haven't seen the output yet (destaining still in progress) but I sure hope it's better than the last. We'll see on Monday. 


Am not labbing tomorrow. Holiday. Long weekend ahead but I will keep myself busy by dehulling mungbeans. Mmmm... fun! 


Short post much? Blame this major headache!

Day Two: The Bowling Alley Phenomenon

Of the 24 hours of Day Two, nine hours were spent in the lab. First thing I did was to check on the now-completely-destained electrophoretogram from our first SDS-PAGE attempt (see Day One). What it looked like? Hmmm, let's just say that we have discovered a new electrophoretogram phenomenon. The people in the lab called it The Bowling Alley Phenomenon. Here's a snippet of the actual electrophoretogram.

Bowling Alley Phenomenon, get it?


Anyway... Today's agenda: SDS-PAGE attempt #2 and Bradford assay attempt #... uhm... Bradford assay, period. Bradford assay, quite successful ---> proof that, indeed, practice makes perfect. Our assay was not yet perfect, though. But we're getting there. :) SDS-PAGE? Failure (yet again). But as I've said, practice makes perfect. We'll get there sometime. :)


Tonight I'm gonna resume my Heroes Season 4 marathon. Or maybe I'll just finish reading Percy Jackson: The Sea of Monsters. Whichever I choose to do, I'll do it while eating Bavarian-flavored donuts.


Tomorrow's agenda: SDS-PAGE attempt #3 and summer 2010 registration.