Day 24: Frustrations

You know what they say... "Nothing is perfect." And my work in the laboratory is no exception. I just have to accept that we don't always get what we want when we want them. We don't always get favorable results on the first try, right? These things... they take time. And that's the sad part. Time is what I don't have right now. And I am even starting to think that maybe 30 days aren't enough. I know it's my own fault. I let myself be distracted by so many things back when I had all the time in the world. So now I am in so much pressure. Maybe this is the reason why I've been acting kind of weird lately. I feel so angry and irritable most of the time. I vent out my frustrations to innocent people. I'm becoming more and more sensitive and emotional. I know all this will be over. I just hope it will all be over soon.

I've got no time to lose. Next week's gonna be one hell of a week for me, I know it. I won't even get to rest and enjoy on my own birthday. Well, that sucks.

Day Two: The Bowling Alley Phenomenon

Of the 24 hours of Day Two, nine hours were spent in the lab. First thing I did was to check on the now-completely-destained electrophoretogram from our first SDS-PAGE attempt (see Day One). What it looked like? Hmmm, let's just say that we have discovered a new electrophoretogram phenomenon. The people in the lab called it The Bowling Alley Phenomenon. Here's a snippet of the actual electrophoretogram.

Bowling Alley Phenomenon, get it?


Anyway... Today's agenda: SDS-PAGE attempt #2 and Bradford assay attempt #... uhm... Bradford assay, period. Bradford assay, quite successful ---> proof that, indeed, practice makes perfect. Our assay was not yet perfect, though. But we're getting there. :) SDS-PAGE? Failure (yet again). But as I've said, practice makes perfect. We'll get there sometime. :)


Tonight I'm gonna resume my Heroes Season 4 marathon. Or maybe I'll just finish reading Percy Jackson: The Sea of Monsters. Whichever I choose to do, I'll do it while eating Bavarian-flavored donuts.


Tomorrow's agenda: SDS-PAGE attempt #3 and summer 2010 registration.